TECHNOLOGIES FOR VIRAL VECTORS & VACCINES

Designer Cell Line AGE1.CR.plX^®

If you're looking for a high performing avian cell line for vaccine viruses, our immortalized and stable proliferating avian cell line AGE1.CR.pIX^® is your ideal solution.

The cell line is fully developed under GMP-compliance and is completely documented.

IN A NUTSHELL

Highly Permissive Designer Cell Line for Vaccine Viruses

ProBioGen has designed and developed an immortalized and stable proliferating avian cell line, the AGE1.CR.pIX^®, that is highly permissive for a variety of different vaccine viruses, including poxvirus vectors. The cell line development, from egg to generation of cell banks, was performed completely under GMP-compliance at ProBioGen and has been fully documented. We developed a chemically-defined medium that allows optimal growth. The cell line is available both as an adherent and as a suspension cell line and is well known in the academic and industrial community.

Key Features of AGE1.CR.PIX^®

Fully-documented and free of adventitious agents, including active endogenous retroviruses

Robust and fully-scalable proliferation in suspension culture for different bioreactor systems in chemically-defined media

Highly susceptible to a range of viruses, such as pox-, adeno-, orthomyxo-, rhabdo-, paramyxo- and togaviruses

Readily transferable for flexible, local GMP manufacturing with a small footprint

Cost-saving over manufacturing in CEFs

HOW WE WORK

Suspension USP and Culture Media

The AGE1.CR.pIX^® is adapted for suspension culture. The chemically-defined culture media is also available in a powder format to ensure long shelf life, smart storage, and supply chain features.

Cell Banks and Safety

Master cell banks (MCB) and working cell banks (WCB) are available. Extensive safety testing in vitro and in vivo has revealed no adventitious agents. This safety profile is confirmed by transcriptome analysis against pathogen databases. There are no known or new viral species present in the AGE1.CR.pIX^® cell line.

AGE1 Workhorse

AGE1.CR.pIX^® is a universal platform for a variety of viral vectors and vaccine manufacturing, e. g. gene-therapeutical vectors, oncolytic viruses, and attenuated and vectored vaccines to prevent and cure diseases. It is a key to cost-effective and potent medical interventions.

Use Cases

AGE1.CR.pIX^® has demonstrated highly beneficial vaccine production properties, also beyond MVA. A variety of viruses, viral vectors, and vaccine strains have been shown to replicate in AGE1.CR.pIX^®, including poxviruses such as:

Wildtype and vectored MVA
Fowlpox vectors and veterinary fowlpox virus
Canarypox strain ALVAC
Oncolytic and attenuated vaccinia virus derivatives and vectored vaccines such as chimeric alphavirus vectors (with AGE1.CR.pIX-derived helper cell lines)
Egg drop syndrome virus (EDSV)
Marek's disease virus (MDV) and the vaccine strain HVT
Muscovy duck parvovirus (MDPV)
Influenza A and B strains, also temperature-sensitive live vaccine strains
Infectious bursal disease virus (IBDV)
Infectious bronchitis virus (IBV)
Rabies virus (RABV) and vesicular stomatitis virus (VSV)

And many more.

Our Promise to You

Rely on Fast Timelines

One-Stop-Shop with seamless, integrated Services
Parallel Processes
Efficient project execution
Do it right from the beginning

Get Your High Quality Candidate

Scientific excellence
Deep understanding for molecule / Long + broad experience
Strong Analytics teams and stellar quality management services
Enabling technologies

Have the Flexibility You Need

Modular Offering
Customer Centricity/ Strong Partnership Approach
Experienced Project Managers and efficient decision-making

From Gene to Lead Candidate

VIRUSES WE WORK WITH

A Large Variety of Viruses, Viral Vectors and Vaccine Strains Replicate In AGE1.CR.PIX^®

AGE1.CR.pIX® has been demonstrated highly beneficial vaccine production properties also beyond MVA. A variety of viruses, viral vectors and vaccine strains have been proved to replicate in AGE1.CR.pIX® include poxviruses such as:

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Fowlpox vectors and veterinary fowlpox virus

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Egg drop syndrome virus (EDSV)

Infectious bronchitis virus (IBV)
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Muscovy duck parvovirus (MDPV)
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Influenza A and B strains, also temperature-sensitive live vaccine strains

Infectious bursal disease virus (IBDV)
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Marek's disease virus (MDV) and the vaccine strain HVT
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Rabies virus (RABV) and vesicular stomatitis virus (VSV)
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Oncolytic and attenuated vaccinia virus derivatives, vectored vaccines such as chimeric alphavirus vectors

Infographic

Our Promise To You

Highly Permissive Cell Line

For a large variety of viruses
For both human and animal vaccine production, and vectors

Highly Evolved Package

Chemically-defined media optimized for cell line, also in powder format
Adherent and suspension cultivation possible
Comprehensive technology transfer for production at desired scale and purpose

Full Documentation

From origin, treatments, passaging and raw materials; we confirm that MCBs/WCBs are free of adventitious agents and any reverse transcriptase activity
Fully GMP compliant (EU and US guidelines)